CHARACTERIZATION OF A NOVEL RESISTANCE-BREAKING ISOLATE OF POTATO VIRUS Y IN NICOTIANA TABACUM

Jae-Hyun Kim, Young-Soo Kim, Soo-Won Jang, Yong-Ho Jeon

Abstract


Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea. Biological properties of the isolate named PVY-ToBR1 was characterized using various host plants with another isolate PVY-ToJC37. The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed. By contrast, the isolate PVY-ToJC37 failed to infect VAM plants and the virus was not detected on inoculated leaf and systemic leaves in VAM plants. Similarly, the isolated PVY-ToBR1 induced distinctly systemic vein necrosis symptoms on PVY-resistant tobacco cultivars (V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislica) that have the recessive potyvirus resistance gene va, but PVY-ToJC37 did not infect systemically infect these tobacco cultivars, suggesting that PVY-ToBR1 is a novel resistance-breaking isolate in tobacco. The coat protein (CP) genes of PVY-ToBR1 and PVY-ToJC37 were amplified using RT-PCR assays with specific primers for PVY isolates and nucleotide sequences of the CP genes were determined. The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively. Phylogenetic relationship from CP comparisons showed that PVY-ToBR1 isolate clustered with PVYNTN isolates and PVY-ToBR1 isolate more closely related to the isolates from European than from North American PVY NTN.


Keywords


Coat protein, identification, potato virus Y, potyvirus, resistance, tobacco

References


Acosta-Leal, R. 1999. A plant resistance mechanism that promotes the emergence of resistance-breaking variants of potato Y potyvirus.

Beczner, L., J. Horváth, I. Romhanyi, H. Förster. 1984. Studies on the etiology of tuber necrotic ringspot disease in potato. Potato Research. 27. 339-352.

Burk, L., J. Chaplin, G. Gooding, N. Powell. 1979. Quantity production of anther-derived haploids from a multiple disease resistant tobacco hybrid. I. Frequency of plants with resistance or susceptibility to tobacco mosaic virus (TMV), potato virus Y (PVY), and root knot (RK). Euphytica. 28. 201-208.

Chaplin, J., L. Burk, G. Gooding, N. Powell. 1980. Registration of NC 744 tobacco germplasm (Reg. No. GP18). Crop Science. 20.

De Bokx, J., H. Huttinga. 1981. Potato virus Y. CMI/AAB Descriptions of plant viruses. 242. 6pp.

Dolja, V., R. Haldeman, N. Robertson, W. Dougherty, J. Carrington. 1994. Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants. The EMBO journal. 13. 1482.

Dolja, V.V., R. Haldeman-Cahill, A.E. Montgomery, K.A. Vandenbosch, J.C. Carrington. 1995. Capsid protein determinants involved in cell-to-cell and long distance movement of tobacco etch potyvirus. Virology. 206. 1007-1016.

Fischer, D., R. Rufty. 1993. Inheritance of partial resistance to tobacco etch virus and tobacco vein mottling virus in burley tobacco cultivar Sota 6505. Plant disease. 77. 662-666.

García-Arenal, F., A. Fraile, J.M. Malpica. 2001. Variability and genetic structure of plant virus populations. Annual review of phytopathology. 39. 157-186.

Gibb, K.S., G.M. Hellmann, T.P. Pirone. 1989. Nature of resistance of a tobacco cultivar to tobacco vein mottling virus. Mol. Plant-Microbe Interact. 2. 332-339.

Gooding Jr, G., G. Kennedy. 1985. Resistance in tobacco breeding line NC 744 to potato virus Y and inoculation by aphids. Plant disease (USA).

Gupton, C. 1980. Registration of Greeneville 107 Burley tobacco germplasm (Reg. No. GP17). Crop Science. 20.

Gupton, C., L. Burk. 1973. Location of the factor for resistance to potato virus Y in tobacco. Journal of heredity. 64. 289-290.

Harrison, B.D. 2002. Virus variation in relation to resistance-breaking in plants. Euphytica. 124. 181-192.

Holt, C. 1992. Detection and localization of plant pathogens. Tissue Printing: Tools for the Study of Anatomy, Histochemistry and Gene Expression. PD Reid and RF Pont-Lezica, eds. Academic Press, San Diego, CA. 125-137.

Johnson, M., T. Pirone. 1982. Evaluation of Tobacco Introduction 1406 as a source of virus resistance [Cultivar mutant]. Phytopathology.

Jung, J.U., J.-K. Choi, A. Ensser, B. Biesinger. 1999. Herpesvirus saimiri as a model for gammaherpesvirus oncogenesis. Seminars in cancer biology. Elsevier. pp. 231-239.

Jung, J.U., M. Stäger, R.C. Desrosiers. 1994. Virus-encoded cyclin. Molecular and cellular biology. 14. 7235-7244.

Keum, W., Y. Chung, S. Jung, S. Choi, S. Lee. 1991. Inheritance of resistance to potato virus Y vein-necrosis strain of N. Africana. Journal of the Korean Society of Tobacco Science (Korea Republic).

Koelle, G. 1958. Versuche zur Vererbung der Krankheitsresistenz bei Tabak. III. Mitteilung: Versuche zur Resistenz gegenüber Rippenbräune in den Jahren 1954 bis 1957. Tabak-Forschung. 25. 90-92.

Latorre, B., V. Flores. 1985. Strain identification and cross-protection of potato virus Y affecting tobacco in Chile. Plant disease. 69.

Le Romancer, M., C. Kerlan. 1991. La maladie des nécroses annulaires superficielles des tubercules: une affection de la pomme de terre, due au virus Y. Agronomie. 11. 889-900.

Le Romancer, M., C. Kerlan, M. Nedellec. 1994. Biological characterisation of various geographical isolates of potato virus Y inducing superficial necrosis on potato tubers. Plant Pathology. 43. 138-144.

Lewis, R. 2005. Transfer of resistance to potato virus Y (PVY) from Nicotiana africana to Nicotiana tabacum: possible influence of tissue culture on the rate of introgression. Theoretical and applied genetics. 110. 678-687.

Lucas, G. 1975. Diseases of Tobacco (3rd edn.) Biological Consulting Associates. Raleigh, USA.

Masuta, C., M. Nishimura, H. Morishita, T. Hataya. 1999. A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breaking in'Virgin A Mutant'tobacco. Phytopathology. 89. 118-123.

McDonald, J., R. Singh. 1996. Response of potato cultivars to North American isolates of PVYNTN. American potato journal. 73. 317-323.

Miller, R. 1987. Registration of'TN 86'burley tobacco. Crop Science. 27. 365-366.

Miller, R. 1991. Registration of'TN90'burley tobacco. Crop Science. 31.

Nicolas, O., S.W. Dunnington, L.F. Gotow, T.P. Pirone, G.M. Hellmann. 1997. Variations in the VPg Protein Allow a Potyvirus to Overcome< i> va Gene Resistance in Tobacco. Virology. 237. 452-459.

Nicolas, O., T. Pirone, G. Hellmann. 1996. Construction and analysis of infectious transcripts from a resistance-breaking strain of tobacco vein mottling potyvirus. Archives of virology. 141. 1535-1552.

Przybys, M., T. Doroszewska, A. Berbec. 2013. Point mutations in the viral genome-linked protein (VPg) of Potato virus Y probably correspond with ability to overcome resistance of tobacco. International journal of food, agriculture and environment. 11. 986-989.

Riechmann, J.L., S. Laín, J.A. García. 1992. Highlights and prospects of potyvirus molecular biology. Journal of General Virology. 73. 1-16.

Robaglia, C., M. Durand-Tardif, M. Tronchet, G. Boudazin, S. Astier-Manifacier, F. Casse-Delbart. 1989. Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology. 70. 935-947.

Roberts, A.G., S.S. Cruz, I.M. Roberts, D.A. Prior, R. Turgeon, K.J. Oparka. 1997. Phloem unloading in sink leaves of Nicotiana benthamiana: comparison of a fluorescent solute with a fluorescent virus. The Plant Cell Online. 9. 1381-1396.

Rojas, M.R., F.M. Zerbini, R.F. Allison, R.L. Gilbertson, W.J. Lucas. 1997. Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology. 237. 283-295.

Sambrook, J., E.F. Fritsch, T. Maniatis. 1989. Molecular cloning. Cold spring harbor laboratory press New York.

Shukla, D.D., C.W. Ward, A.A. Brunt. 1994. The Potyviridae. Cab International.

Stavely, J. 1979. Disease resistance. Nicotiana: procedures for experimental use. USDA Technical Bulletin. 1586. 87-110.

Van den Heuvel, J., R. Van der Vlugt, M. Verbeek, P. De Haan, H. Huttinga. 1994. Characteristics of a resistance-breaking isolate of potato virus Y causing potato tuber necrotic ringspot disease. European Journal of Plant Pathology. 100. 347-356.

Weidemann, H. 1988. Importance and control of potato virus YN (PVYN) in seed potato production. Potato Research. 31. 85-94.

Wernsman, E. 1992. Varied roles for the haploid sporophyte in plant improvement. Plant breeding in the 1990s. CAB International. pp. 461-484.


Full Text: PDF

Refbacks

  • There are currently no refbacks.




Copyright (c) 2014 Jae-Hyun Kim, Young-Soo Kim, Soo-Won Jang, Yong-Ho Jeon

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.