Isolation, Identification and Biomanagement of Root Rot of Black Cumin (Nigella sativa) Using Selected Bacterial Antagonists

Al-Sman K. Mohamed, Abo-El-yousr A. M. Kamal, Eraky Amal, El-Zawahry Aida

Abstract


The study deal with potentiality of some bioagents for controlling the root rot of black cumin under greenhouse conditions, caused by Fusarium spp. Eight fungal isolates were obtained from diseased of back cumin plants collected from Assiut Governorate. These isolates were belonged to the genus Fusarium spp. They were identified as, four isolates of F. comptoceras, three isolates of F. solani and one isolate Fusarium lateritium. Pathogenicity tests indicated that all tested fungal isolates were able to infect black cumin plants causing symptoms of root rot resulted in dwarfism and death before the capsules mature. They varied in their pathogenicity, Fusarium comptoceras No.1 gave the highest percentage of disease severity and percentage of infection on black cumin plants (53 and 50% respectively), while isolates F. comptoceras Nos. 3 and F. solani No. 6 gave the lowest percentage of infection (15 and 17% respectively) the rest of isolates showed moderate of percentage of infection. Antagonistic capability of 15 isolates (PGPR) was tested in vitro against growth of three isolates of Fusarium spp. the causal pathogen of root rot of black cumin. Seeds black cumin plant treated with all bioagents as a suspension significantly increased the root dry weigh and foliar dry weigh compared to infected control. In conclusion, our study confirmed that used of bioagents may be applied as future ecofriendly alternatives to synthetic fungicides for controlling the disease of black cumin.


Keywords


Fusarium; Root rot; Black cumin; Rhizobacteria; Number of colony fungi

References


Aneja, K. R. 2003. Experiment in Microbiology, Plant Pathology and Biotechnology. New Age International (P) Limited, Publishers. New Delhi, 157-161.

Booth, C. 1971. The genus Fusarium common weal the Mycological institute, New surrey, England. Pp. 237.

Brown, W. 1924. Tow mycological methods a method of isolation singl strain of fungi by cutting a hyphal tip: Annual Botony, 38: 404.

Chang, Y. C., C. Yih, R. Chang, O. Baker-Kleifeld and I. Chet. 1986. Increased growth of plant in the presence of the biological control agent Trichodema harzianum. Plant Diseases, 70: 145-148.

Cook, R. J. and K. F. Baker. 1983. The Nature and Practice of Biological Control of Plant Pathogens.American Phytopathol. Soc., St. Paul, Minnesota, p.539.

Dashti, N., F. Zhang, R. Hynes and D. L. Smith. 1998. Plant growth promoting rhizobacteria accelerate nodulation and increase nitrogen fixation activity by field grown soybean (Glycine max (L.) Merr.) under short season conditions. Plant Soil, 200: 205–213.

Deshwal, V. K. and P. Kumar. 2013. Effect of heavy metals on growth and PGPR activity of Pseudomonads. Production of plant growth promoting substance by Pseudomonads. Journal Acadamic Indus Research, 2(5): 286-290.

Dhingra, O. D. and J. B. Sinclair. 1995. Basic plant pathology methods. Second Edition. Lewis Publishers, CRC press, USA, 400-450.

Domsch K. H., W. Gams and T. H. Anderson. 1980. Compen¬dium of Soil Fungi. London, Academic Press.

Dubey, S. C, M. Suresh and B. Singh. 2007. Evaluation of Trichoderma species against Fusarium oxysporum. f. sp. ciceris for integrated management of chickpea wilt., Biological Control, 40: 118-127.

Dye, D. W. 1968. Ataxonomic study of the genus Erwinia. I. the "amylovora" group. New Zealand Journal of Science, 11: 590-607.

El-Degwy, A. 2004. Encyclopedia of growing and production of ornamental plants landscape and flowers 258–268. Madboly Library Publishing Company.

Gabr, M. R., O. I. Saleh, N. A. Hussein and M. A. Khalil. 1998. studies and cell wall degrading enzymes of Fusarium oxysporium f. sp. sesame and Macrophomina phaseolina: The causal organisms of wilt and root rot diseases of sesame. Egyptian Journal of Microbiology, 33: 595-610.

Gams, W. 1971. Cephalosporium-artige Shimmelpilze (Hyphomycetes). Gustav Fisher Verlag. New York, 262 pp.

Gilman, J. C. 1957. Manual of soil fungi lowa State University Press USA. pp 1-450.

Goksel, O. and B. Harun. 2015. Determination of Fungal Pathogens Associated with Cuminum cyminum in Turkey. Plant Protect. Sci., 51: 74–79.

Gomez, K. A. and A. A. Gomez. 1984. Statistical Procedures for Agriculture Research, second ed. Wiley, New York, pp 680.

Hilal, A. A. 1985. Pathological studies on some medicinal and aromatic plants in Egypt. Ph.D. Thesis, Faculty of Agriculture Suez Canal University. pp 1-236.

Hoda, H. El-Hendawy and K. A. M. Abo-Elyousr. 2016. Combination of different antagonistic bacteria to control of potato blackleg disease caused by Pectobacterium atrosepticum under greenhouse and field conditions. International Journal of Phytopathology, 5(1): 35-43.

Holt, J. G. N. R. Krieg, P. H. A. Sneathm, J. T. Staley and S. T. Williams. 1994. Bergey's Manual of Determination Bacteriology 9thEds Williams and Wilkinc, Baltimore, Maryland, U.S.A. 787.

Idris, E. E. S., R. H. Bochow and R. Borriss. 2004. Use of Bacillus subtilis as biocontrol agent. VI. Phytohormone like action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45 and Bacillus subtilis FZB37. Journal of Plant Diseases and Protection, 111: 583-597.

Jetiyanon, K. and J. W. Kloepper. 2002. Mixtures of plant growth- promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biological Control, 24: 285–291.

Koike, S. T. 2005. First Report of Fusarium Wilt of Cilantro Caused by Fusarium oxysporum in California. Plant Disease, 89: 10 1, 130.

Kumar, B. S. D., I. Berggren and A. M. Martensson. 2001. Potential for improving pea production by co-inoculation with fluorescent Pseudomonas and Rhizobium. Plant Soil, 229: 25–34.

Linderman, R. G. 1994. Effects of biocontrol agents on plant growth. The Intl. Plant Propagators Society: Combined Proceedings, 43: 249-252.

Mohammadi, A. and N. N. Mofrad. 2009. Genetic diversity in population of Fusarium solanifrom cumin in Iran. Journal of Plant Protection Research, 49: 283–286.

Mohmed, E. F., M. I. El-Refaei, A. A. Hial and A. G. Abdel-Wahed. 2012. Using of Tissue Culture to Dectease the Deterioration of Productivity of Pelargonium Graveolenes Plant in Egypt. Researcher, 4: 16.

Nelson, P. E., T. A. Toussoun and W. F. O. Marasas. 1983. Fusarium species: An illustrated Manual for Identification. Pennsylvania State University Press, University Park.193pp.

Pastrova, A., M. Ropk and A. Asova. 2004. salicylic acid induced changes of comarin metabolites in Matricaria chamomilla. Plant Science, 167: 819-824.

Ramchandra, S. and P. N. Bhatt. 2012. First report of Fusarium equiseti causing vascular wilt of cumin in India. Plant Disease, 96: 1821.

Reuveni, R. 1982. Fusarium equiseti a new cause of cumin spice plant wilt in Israel. Plant Disease, 66: 498–499.

Sallam-Nashwa, M., N. Riad-Shaimaa, M. Mohamed and A. Seef-Eleslam. 2014. Biocontrol of cantaloupe damping-off disease caused by Fusarium semitectum by using formulations of antagonistic fungi. Journal of Phytopathology and Pest Management, 1(1): 5-15.

Schaad, N. W. 1988. Identification schemes, p. 1-15. In: N.W. Schaad (ed.). Laboratory guide of identification of plant pathogenic bacteria.2nd ed. Amer. Phytopathological Soc. Press, St. Paul, Minn.

Seleim, M. A. A., F. A. Saead, K. M. H. Abd-El-Moneem and K. A. M. Abo-ELyousr. 2011. Biological control of bacterial wilt of tomato by plant growth promoting rhizobacteria. Plant Pathology Journal, 10: 146-153.

Sharma, Y. K. and S. S. Meena. 2012. Effect of planting time and plant spacing on root rot of Nigella sativa. International Journal of Seed Spices, 2(1): 77-78.

Shoda, M. 2000. Bacterial Control of Plant Diseases. Journal Bioscience and Bioengineering, 89: 515-521.

Siddiqui, Z. 2006. PGPR: Prospective Biocontrol Agents of Plant Pathogens. Biocontrol. Biofertil. pp. 111-142.

Tawfik, Azza, A. and A. D. Allam. 2004. Improving cumin production under soil infestation with Fusarium wilt pathogen. I- Screening of biocontrol agents. Ass. Univ. Bull. Environ. Res. Assiut, Egypt, 7 (2): 35-45.

Tinline, R. D., R. J. Ledingham and B. J. Sallans. 1975. Appraisal of loss from common root in wheat. In: Bruehl G.W. (ed.). Biology and Control of Soil-borne Plant Pathogens, pp. 22-26.

Zdravkovic, J., M. Ugrinovic, M. Zdravkovic, S. Djordjevic, S. Pavlovic and D. Josic. 2015. In vitro and in vivo effects of Pseudomonas spp. and Bacillus sp. on Fusarium acuminatum, Botrytis cinerea and Aspergillus niger infecting cucumber. Pestic. Phytomed. (Belgrade), 30(3): 169–178.

Zhang, F., N. Dashti, R. K. Hynes and D. L. Smith. 1996. Plant growth promoting rhizobacteria and soybean (Glycine max (L.) Merr.) nodulation and nitrogen fixation at suboptimal root zone temperatures. Annaual Botony, 77: 453–459.


Full Text: PDF XPS

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Al-Sman K. Mohamed, Abo-El-yousr A. M. Kamal, Eraky Amal, El-Zawahry Aida

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.