Elicitation of Phytoalexin rishitin in Potato Tuber Slices Infected by Fusarium spp., Does it Consider a Factor of Pathogenicity?

Mostafa H. Mostafa

Abstract


Potato tubers, under storage conditions, suffer from Fusarium dry rot disease caused by Fusarium spp. leading to devastating losses of stored tubers.  Newly harvested tubers are resistance toward Fusarium infection, but disease becomes more vigorous by increasing storage period.  Inoculation of potato tuber slices of Desiree cv. (highly susceptible) or Spunta cv. (highly resistant) either by a weak pathogen (F. moniliforme) or severe pathogen (F. solani) and tracking accumulation of phytoalexin rishitin in inoculated tissues at different intervals from inoculation revealed that Desiree cv. accumulated high amounts of rishitin in a very short time in comparison to another three tested situations. Dipping of potato tuber slices of both cvs. in different concentrations of the known antibiotic chloramphenicol prior to inoculation with a weak pathogen (F. moniliforme) led to increased disease severity associated with accumulation huge amounts of rishitin in inoculated tissues. The correlation coefficient between disease severity and rishitin production was +0.83.  Moreover, the reaction of spunta cv. toward inoculation by a weak pathogen was changed from the state of resistance to very severe susceptibility.  Since chloramphenicol affects protein synthesis on 70S ribosome’s, it was postulated that it prevents synthesis of factor(s) take a part in cell death during infection. Mitochondria extracted from potato tuber tissues secret compound(s) decreased rishitin synthesis in potato tuber slices treated with mycelia extract of F. solani. Treatment of isolated mitochondria with this antibiotic led to the elimination of this factor(s) from their secretions. Results obtained revealed that the elicitation of phytoalexins may consider one of pathogenicity factor of such system.


Keywords


Fusarium moniliforme; Fusarium solani; Spunta cv.; Desiree cv.; Chloramphenicol; mitochondria; dry rot; phytoalexin

References


Allen, E. and J. Kuc. 1964. Steroid alkaloids in disease resistance of white potato tubers Phytopathology. American Phytopathological Society 3340 Pilot Knob Road, ST Paul, MN 55121.

Alves, L. M., E. G. Heisler, J. C. Kissinger, J. M. Patterson and E. B. Kalan. 1979. Effects of controlled atmospheres on the production of sesquiterpenoid stress metabolites by white potato tuber: Possible involvement of cyanide-resistant respiration. Plant Physiology, 63: 359-62.

Ashour, W. and M. Mostafa. 1983. Studies on the interaction between potato tuber tissues and Fusarium solani. I. Phenylalanine ammonia lyase, orthodihydroxy phenol oxidase, soluble peroxidase and cell wall hydrolyzing enzymes activities. Annals of agricultural science.

Bostock, R. M. 1983. Effects of Potato Tuber Age and Storage on Sesquiterpenoid Stress Metabolite Accumulation, Steroid Glycoalkaloid Accumulation, and Response to Abscisic and Arachidonic Acids. Phytopathology, 73: 435.

Corsini, D., J. Pavek and G. Fenwick. 1977. Characterization of Fusarium dry rot resistance in potato breeding clones. Proceedings American Phytopathological Society.

Corsini, D. L. and J. J. Pavek. 1980. Phenylalanine ammonia lyase activity and fungitoxic metabolites produced by potato cultivars in response to Fusarium tuber rot. Physiological Plant Pathology, 16: 63-72.

Coutsogeorgopoulos, C. 1966. On the mechanism of action of chloramphenicol in protein synthesis. Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 129: 214-17.

Darvill, A. G. and P. Albersheim. 1984. Phytoalexins and their Elicitors-A Defense Against Microbial Infection in Plants. Annual Review of Plant Physiology, 35: 243-75.

Desjardins, A. E., H. W. Gardner and R. D. Plattner. 1989. Detoxification of the potato phytoalexin lubimin by Gibberella pulicaris. Phytochemistry, 28: 431-37.

Desjardins, A. E., H. W. Gardner and K.-M. Weltring. 1992. Detoxification of sesquiterpene phytoalexins byGibberella pulicaris (Fusarium sambucinum) and its importance for virulence on potato tubers. Journal of Industrial Microbiology, 9: 201-11.

Dische, Z. 1962. Colour reactions of carbohydrates. Methods in carbohydrate chemistry, 1: 477-512.

El-Hassan, K., M. El-Saman, A. Mosa and M. Mostafa. 2007. Variation among Fusarium spp. the causal of potato tuber dry rot in their pathogenicity and mycotoxins production. Egyptian Journal of Phytopathology, 35: 53-68.

Ersek, T., B. Barna and Z. Kiraly. 1973. Hypersensitivity and the resistance of potato tuber tissues to Phytophthora infestans. Acta phytopathologica.

Ghahramani, S. 2000. Fundamentals of probability Prentice Hall New Jersey:.

Ingham, J. L. 1972. Phytoalexins and other natural products as factors in plant disease resistance. The Botanical Review, 38: 343-424.

Kenney, F. and E. Keeping. 1951. Mathematics of statistics-part two.

Kistler, H. C. and H. D. VanEtten. 1981. Phaseollin metabolism and tolerance in Fusarium solani f. sp. phaseoli. Physiological Plant Pathology, 19: 257-71.

Kroon, A. M. 1965. Protein synthesis in mitochondria III. On the effects of inhibitors on the incorporation of amino acids into protein by intact mitochondria and digitonin fractions. Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 108: 275-84.

Laval-Martin, D. and P. Mazliak. 1979. Plant physiology, practical works and planned works.

Leach, S. S. 1981. Resistance of selected potato cultivars and clones to fusarium dry rot. Phytopathology, 71: 623.

Li, C.-H., S.-L. Tzeng, Y.-W. Cheng and J.-J. Kang. 2005. Chloramphenicol-induced Mitochondrial Stress Increases p21 Expression and Prevents Cell Apoptosis through a p21-dependent Pathway. Journal of Biological Chemistry, 280: 26193-99.

Lulai, E. C. and D. L. Corsini. 1998. Differential deposition of suberin phenolic and aliphatic domains and their roles in resistance to infection during potato tuber (Solanum tuberosumL.) wound-healing. Physiological and Molecular Plant Pathology, 53: 209-22.

Lyon, G. D. 1980. Evidence that the toxic effect of rishitin may be due to membrane damage. Journal of Experimental Botany, 31: 957-66.

McCue, K. F. 2009. Potato glycoalkaloids, past present and future. Fruit, Vegetable and Cereal Science and Biotechnology, 3.

McKee, R. K. 1959. Factors affecting the toxicity of solanine and related alkaloids to Fusarium caeruleum. Journal of General Microbiology, 20: 686-96.

Mejdoub, T. B., H. J. Khiareddine and M. D. Remadi. 2015. Interactions between four Fusarium species in potato tubers and consequences for fungal development and susceptibility assessment of five potato cultivars under different storage temperature. Journal of Plant Pathology and Microbiology, 06: 1-12.

Pathuri, I. P., N. Zellerhoff, U. Schaffrath, G. Hensel, J. Kumlehn, K.-H. Kogel, R. Eichmann and R. Hückelhoven. 2008. Constitutively activated barley ROPs modulate epidermal cell size, defense reactions and interactions with fungal leaf pathogens. Plant Cell Reports, 27: 1877-87.

Paxton, J. D. 1981. Phytoalexins-A working redefinition. Journal of Phytopathology, 101: 106-09.

Stoessl, A., J. B. Stothers and E. W. B. Ward. 1976. Sesquiterpenoid stress compounds of the Solanaceae. Phytochemistry, 15: 855-72.

Suzuki, A., Y. Tsutomi, N. Yamamoto, T. Shibutani and K. Akahane. 1999. Mitochondrial regulation of cell death: Mitochondria are essential for procaspase 3-p21 complex formation to resist Fas-mediated cell death. Molecular and Cellular Biology, 19: 3842-47.

VanEtten, H. 1982. Phytoalexin detoxification by monooxygenases and its importance for pathogenicity. Plant Infection: The Physiological and Biochemical Basis.

Yang, N. S. and J. G. Scandalios. 1977. Effects of cycloheximide and chloramphenicol on the synthesis of polypeptides found in three subcellular fractions of maize scutellum. Plant Physiology, 59: 1067-71.


Full Text: PDF XPS

DOI: 10.33687/phytopath.007.02.2383

Refbacks

  • There are currently no refbacks.




Copyright (c) 2018 Mostafa H. Mostafa

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.