COMPLETE CHLOROPLAST GENOME SEQUENCE OF ASPARAGUS (ASPARAGUS OFFICINALIS L.) AND ITS PHYLOGENETIC POSITION WITHIN ASPARAGALES

Wentao Sheng, Xuewen Chai, Yousheng Rao, Xutang Tu, Shangguang Du

Abstract


Asparagus (Asparagus officinalis L.) is a horticultural plant with health care, which is meaningful to sequence the entire chloroplast (cp) genome of asparagus with Hiseq4000 platform. The complete cp genome maps a circular molecule of 156,699bp built with a quadripartite organization: two inverted repeats (IRs) of 26,531bp, separated by a large single copy (LSC) sequence of 84,999bp and a small single copy (SSC) sequence of 18,638bp. A total of 112 genes comprising of 78 protein-coding genes, 30 tRNAs and 4 rRNAs were successfully annotated, 17 of which included introns. The identity, number and GC content of asparagus cp genes were similar to those of other asparagus species genome. Analysis revealed 81 simple sequence repeat (SSR) loci, most composed of A or T, contributing to a bias in base composition. A maximum likelihood of phylogenomic evolution analysis showed that asparagus was closely related to Polygonatum cyrtonema that belonged to the genus Asparagales.The availability of the complete cp genome sequence of asparagus provides valuable information for chloroplast genetic engineering and phylogenetic analyses in Asparagales.


Keywords


Asparagus officinalis L.; Chloroplast genome; Phylogenomic evolution; Asparagales

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